Optomicrofluidic measurement of particle-encapsulated droplet system

TL;DR

This study explores how optical and geometrical parameters affect fluorescence detection in droplet microfluidics, revealing optimal conditions for sensitivity and uniformity in single-cell assays.

Contribution

It provides a comprehensive analysis combining experiments and simulations to optimize particle-encapsulated droplet detection in optomicrofluidic systems.

Findings

PRs are prominent when particle-to-droplet size ratio is 0.23-0.33

Particles near the droplet center yield more uniform signals

Reducing oil layer thickness enhances fluorescence intensity

Abstract

Droplet microfluidics combined with optical detection has become a powerful approach for high-throughput single-cell assays, but these systems often face limited sensitivity and signal heterogeneity due to optical and geometrical constraints. We investigate how key operating parameters influence the performance of a droplet-based optomicrofluidic platform. Experiments examine optical interactions between guided light and aqueous droplets containing fluorescent (FL) particles flowing in oil. Geometrical optics simulations model light-droplet interactions, while FL simulations quantify signal variations caused by particle size and position. Two refracted signals are observed experimentally: a droplet-refracted signal (DRS) that scales with droplet diameter and a particle-refracted signal (PRS) produced by light interaction with encapsulated particles. Both experiments and simulations show that PRS becomes prominent when the particle-to-droplet size ratio $D^*_\text{p}$ lies between 0.23-0.33, enabling label-free detection. Particles near the droplet center ($r^*_\text{p} < 0.4$) display reduced angular dependence and more uniform FL signals. Simulations further show that FL intensity increases with $D^*_\text{p}$, rising sharply from 0.33 to 0.5 and more gradually up to 0.66. Additionally, reducing the oil layer thickness enhances fluorescence by minimizing optical losses at the droplet-channel interface. These results demonstrate that controlling $D^*_\text{p}$, particle position, and oil layer thickness improves FL strength and uniformity, providing a framework for optimizing droplet-based fluorescence detection in microflow cytometry and single-cell assays.