Evaporation-Induced Pattern Formation and Wetting in Active Microtubule-Kinesin Droplets

TL;DR

This study investigates how evaporation influences active microtubule-kinesin droplets, revealing how active stresses and flows lead to unique pattern formation and wetting behaviors in biomimetic systems.

Contribution

It combines experimental and theoretical methods to elucidate the role of motor protein crosslinking and evaporation in active network dynamics and wetting phenomena.

Findings

Capillary and Marangoni flows induce radial microtubule organization.

Motor protein activity causes non-monotonic wetting behavior.

Active stresses influence contact-line dynamics during evaporation.

Abstract

Active networks composed of biopolymers and motor proteins provide versatile biomimetic systems that have advanced active matter physics and deepened our understanding of cytoskeletal dynamics and self-organization under diverse stimuli. In these systems, activity arises in aqueous solutions where motor proteins cross-link biopolymers and generate active stress driving the emergent network behavior. Here, we establish the active network in the form of a sessile, multi-component droplet on a substrate and investigate how evaporation influences its dynamics. We focus on how mass loss and compositional changes in the droplet reshape the behavior of the active suspension. We show that capillary and Marangoni flows drive the self-organization of microtubules into a distinctive radial arrangement within the droplet. The cross-linking ability of motor proteins gives rise to a striking non-monotonic wetting behavior, where the extensile stresses generated by the motor proteins strongly affect the characteristic timescale of the contact-line retracting and subsequent expansion. Using a combined experimental and theoretical approach, we demonstrate the crucial role of crosslinking in evaporating microtubule networks, and explain how active stresses together with evaporation-induced flows govern the dynamics of reconstituted microtubule systems and their wetting behavior. Evaporating droplets have recently attracted significant attention in the scientific community, and the findings of the setup presented in this study can have broad implications, ranging from self-organization and mechanical pattern formation in biological systems to questions about the origin of life.