Microscopy of Bioelectric Potentials using Electrochromism

TL;DR

This paper introduces an advanced electrochromic optical recording (ECORE) method using a high-NA microscope to noninvasively measure bioelectric signals from cells with high sensitivity and resolution, enhancing biological research capabilities.

Contribution

The work develops a high-NA microscope-based ECORE technique that improves sensitivity and resolution for recording cellular bioelectric signals, making the method more accessible and versatile.

Findings

Recorded extracellular action potentials from cardiomyocytes with 3 μV sensitivity.

Achieved single-cell resolution in bioelectric measurements.

Simplified optical setup enables broader research applications.

Abstract

Studying the electrical signals generated by living cells is key to understanding numerous biological phenomena. Electrochromic optical recording (ECORE) uses the electrochromism exhibited by certain materials to noninvasively measure these signals in real time. In this work, we report on the development of ECORE based on a high-NA microscope objective. We demonstrate the recording of extracellular action potentials from cardiomyocytes with single-cell resolution and a high sensitivity of 3 {\mu}V, which compares favorably to the previous record for any ECORE setup. Combining ECORE with microscopy simplifies the optical setup, allows for the simultaneous imaging of specimens, and makes ECORE accessible to a broader community of researchers, allowing for a better understanding of the biological processes that are integral to life.