Nonadiabatic couplings drive ultrafast, mode-selective intramolecular vibrational energy redistribution in flavins

TL;DR

This study reveals how nonadiabatic couplings cause ultrafast, mode-selective vibrational energy redistribution in flavins, affecting their excited state dynamics and potentially influencing biological processes like radical pair formation.

Contribution

It demonstrates the role of nonadiabatic couplings in mode-selective vibrational energy redistribution in flavins using ultrafast vibrational spectroscopy and model calculations.

Findings

High-frequency C-C modes damp within 20 fs

Low-frequency modes persist longer

Nonadiabatic couplings drive energy redistribution

Abstract

Flavins are the chromophores in several blue-light-sensitive photoreceptor proteins and act as redox cofactors in many enzymes relevant for biological processes. Despite their biological relevance and numerous, detailed optical investigations of their photophysical properties, the ultrafast nonequilibrium dynamics of their elementary optical excitations are not yet fully known. Here, we use ultrafast coherent vibrational spectroscopy with 10-fs time resolution in the 450-nm spectral range to study their excited state coherent vibrational dynamics. We observe that coherent wavepacket motion along high-frequency C-C stretching modes with ~ 20-fs period is rapidly damped on a 20-fs timescale. In contrast, coherent motion along several low-frequency modes persists much longer. We attribute this to a mode-selective intramolecular vibrational energy redistribution driven by nonadiabatic couplings between the optical bright state and a close-lying dark electronic state, in accordance with model calculations. Our results may be of relevance for the formation of long-lived radical pair states in magnetic-field sensitive proteins.