Integrated vortex-assisted electroporation platform with enhanced throughput for genetic delivery to primary cells

TL;DR

This paper introduces an integrated vortex-assisted electroporation platform that improves gene delivery efficiency and throughput in primary human cells, addressing heterogeneity and practical application challenges.

Contribution

The study presents a novel, unified electroporation platform combining size-selective cell trapping and optimized parameters for enhanced gene delivery in primary cells.

Findings

Achieved robust plasmid DNA and mRNA delivery in primary human cells.

Enhanced throughput and uniformity in gene delivery compared to conventional methods.

Addressed sample heterogeneity with integrated size-selective trapping.

Abstract

Primary human cells offer the most faithful representation of native human physiology, yet their practical utility is constrained by the difficulty of introducing exogenous genetic material. Electroporation provides a promising non-viral gene delivery approach; however, conventional bulk systems lack the uniformity and integration required for heterogeneous primary cell samples. Here, we present a vortex-assisted electroporation platform integrating size-selective cell trapping with enhanced throughput, parameter optimization across buffer and electrical conditions, and robust delivery of plasmid DNA and in vitro-transcribed mRNA in primary human cells. This integrated platform provides a unified workflow that addresses sample heterogeneity, throughput demands, and delivery efficiency, enabling broader implementation of non-viral gene delivery into primary cells for research and translational applications.