Enzyme-driven phase separation

TL;DR

This paper develops a minimal theoretical model for enzyme-driven phase separation on cell membranes, explaining how non-equilibrium enzymatic activity sustains and controls polarized domain formation, with predictions matching experimental data.

Contribution

It introduces a mesoscopic active Model A framework with explicit phase diagrams and observables derived from reaction kinetics, linking biochemical parameters to phase behavior.

Findings

Phase coexistence is governed by enzymatic activity, not equilibrium saturation.

Domains exchange material rapidly and require continuous energy input.

The model predicts nucleation and coarsening dynamics consistent with simulations.

Abstract

The formation of polarized signaling domains on cell membranes is a fundamental example of biological pattern formation. While such patterns resemble structures from equilibrium phase separation, they are intrinsically non-equilibrium, driven by energy-consuming enzymatic cycles that switch molecules like phosphoinositides or small GTPases between distinct states. Here, we develop a minimal model of this enzyme-driven phase ordering process. Starting from microscopic reaction kinetics, we derive a mesoscopic theory that belongs to the class of active Model A with a global constraint. This framework yields an explicit mean-field phase diagram and closed-form expressions for key observables, such as interfacial tension, domain fractions, and phase coexistence boundaries, in terms of kinetic rates. In this context, phase coexistence is controlled by non-equilibrium parameters like catalytic rates and enzymatic asymmetry, rather than equilibrium parameters such as saturation concentrations. The resulting phase-separated domains rapidly exchange material with their surroundings. Their maintenance requires a continuous power input determined by enzymatic kinetics. The predicted phenomenology is consistent with experimental observations on reconstituted systems of phosphoinositide and Rab5 membrane patterning. We further study how metastable uniform states decay via nucleation of minority-phase domains and subsequent coarsening, driven by an effective interfacial tension. Using large deviation theory, we derive the critical nucleation radius under the action of the intrinsic, multiplicative chemical noise. The analytical results are quantitatively confirmed by stochastic simulations of the process. Our work provides a theoretical framework identifying key biochemical parameters controlling active phase separation on membrane scaffolds, offering testable predictions for experiments.