Nonequilibrium dynamics of membraneless active droplets

TL;DR

This study investigates how active matter, like bacteria, influences liquid-liquid phase separation, leading to stable, motile membraneless droplets with arrested coarsening and unique nonequilibrium behaviors.

Contribution

We developed a bacteria-polymer active phase separation system to explore how active inclusions affect LLPS dynamics, revealing mechanisms for arrested phase separation and droplet stability.

Findings

Active bacteria accelerate initial droplet formation

Droplet coarsening is arrested, leading to stable droplets

Active stresses induce long-wavelength fluctuations and persistent motion

Abstract

Membraneless droplets or liquid condensates formed via liquid-liquid phase separation (LLPS) play a pivotal role in cell biology and hold potential for biomedical engineering. While membraneless droplets are often studied in the context of interactions between passive components, it is increasingly recognized that active matter inclusions, such as molecular motors and catalytic enzymes in cells, play important roles in the formation, transport and interaction of membraneless droplets. Here we developed a bacteria-polymer active phase separation system to study the nonequilibrium effect of active matter inclusions on the LLPS dynamics. We found that the presence of bacterial active matter accelerated the initial condensation of phase-separated liquid droplets but subsequently arrested the droplet coarsening process, resulting in a stable suspension of membraneless active droplets packed with motile bacterial cells. The arrested phase separation of the bacterial active droplet system presumably arises from anti-phase entrainment of interface fluctuations between neighboring droplets, which reduces the frequency of inter-droplet contact and suppresses droplet coarsening. In addition, the active stresses generated by cells within the droplets give rise to an array of nonequilibrium phenomena, such as dominant long-wavelength fluctuations and enhanced droplet transport with short-term persistent motion due to spontaneous symmetry breaking. Our study reveals a unique mechanism for arrested phase separation and long-term stability in membraneless droplet systems. The bacteria-polymer active phase separation system opens a new avenue for studying the dynamics of membraneless active droplets relevant to non-equilibrium LLPS in cells and in biomedical engineering applications.