Tracking phase synchronization between flagella in the time-frequency domain resolves photophobic response

TL;DR

This study uses a novel time-frequency analysis of phase synchronization to understand flagella resynchronization in Chlamydomonas reinhardtii after light shock, revealing distinct swimming stages and signal harmonics.

Contribution

It introduces a wavelet-based phase synchronization index to analyze flagella coordination, uncovering new insights into microalgae response mechanisms.

Findings

Identified three distinct swimming stages with different phase shifts

Detected signal harmonics associated with photoshock response

Showed modulation of spectral beating modes for adaptation

Abstract

The unicellular microalga Chlamydomonas reinhardtii (CR) is well known for its bi-flagellated swimming in response to light stimuli. This work aims to study the resynchronization of CR flagella after a high light intensity stimulus, known as photoshock. The synchronization is estimated thanks to a quantity defined as the Phase Synchronization Index (PSI). The originality of this approach is to perform a time-frequency computation of a complex PSI based on continuous wavelet transform. Thanks to this analysis, we distinguish three swimming stages involving different frequency bands and phase shifts: synchronized breaststroke swimming, undulatory backward swimming, and resynchronization. This approach also reveals the presence of signal harmonics that set the photoshock response, independently of cell variability. Our results suggest that CR modulates the balance between spectral beating modes, providing a mechanism for robust adaptation to sudden environmental stresses.