EEG Dynamic Microstate Patterns Induced by Pulsed Wave Transcranial Photobiomodulation Therapy

TL;DR

This study investigates how pulsed wave transcranial photobiomodulation therapy influences EEG microstate patterns, revealing enhanced brain network efficiency and potential biomarkers for therapy optimization.

Contribution

It demonstrates that pulsed wave tPBM modulates EEG microstates, especially microstate C, and introduces machine learning methods to identify biomarkers for therapy effects.

Findings

PW tPBM improves microstate C network metrics

Microstate features predict therapy mode with high accuracy

EEG microstates may serve as biomarkers for tPBM effects

Abstract

Transcranial photobiomodulation (tPBM) therapy is an emerging, non-invasive neuromodulation technique that has demonstrated considerable potential in the field of neuropsychiatric disorders. Several studies have found that pulsed wave (PW) tPBM therapy yields superior biomodulatory effects. However, its neural mechanisms are still unknown which poses a significant barrier to the development of an optimized protocol. A randomized, single-blind study including 29 participants was conducted using a crossover design, with sham and continuous wave (CW) groups as controls. The EEG microstate analysis was utilized to explore the relative variations in temporal parameters and brain functional connectivity. To further elucidate the dynamic activity patterns of microstates, a 10-repeat 10-fold cross-validation with nine machine learning algorithms and kernel Shapley additive explanations analysis was employed. Results indicated that the pulsed wave mode enhanced the global efficiency, local efficiency, and betweenness centrality of microstate C in brain functional networks as well as the mean durations parameter achieving a middle to large effect size, with superior effects compared to the sham and continuous wave groups. Furthermore, the support vector machine based on the radial basis function method with kernel Shapley additive explanations analysis demonstrated the best performance with an area under the curve (AUC) reaching 0.956, and found that the 8 of top-10 microstate features related to microstate C contributed most significantly to the PW mode. In conclusion, the EEG microstate analysis found that PW tPBM therapy modulates the microstate C-specific patterns in the human brain, suggesting that microstate dynamics may serve as a state-dependent biomarker for the optimization of tPBM protocol.