Light-sheet microscopy to assess cancer pathology: current views and future trends

TL;DR

This paper reviews the use of light-sheet fluorescence microscopy (LSFM) in cancer research, emphasizing its current applications in visualizing tumor tissues and discussing future integration into clinical diagnostics for improved tumor analysis.

Contribution

It provides a comprehensive overview of LSFM's current role in oncology and explores future trends for its clinical adoption in cancer diagnostics.

Findings

LSFM effectively visualizes complex cancer tissue structures.

Potential for high-throughput 3D imaging in clinical settings.

Enhances understanding of tumor architecture.

Abstract

In recent years, light-sheet fluorescence microscopy (LSFM) has emerged as a powerful tool for visualizing and analyzing cancer tissue samples, including patient-derived specimens, organoids, biopsies, and murine models. In this work, we highlight the current applications of deep tissue LSFM in oncology and illustrate its use across a variety of human cancer tissues, including - but not limited to - prostate and breast. Here, we discuss that the potential integration of advanced LSFM technologies into clinical workflows to enable high-throughput, three-dimensional imaging of intact cancer specimens. This approach holds significant potential to enhance diagnostic precision and provide novel insights into tumour architecture and morphology.