Rigid body rotation and chiral reorientation combine in filamentous E. coli swimming in low-Re flows

TL;DR

This study investigates how filamentous E. coli bacteria swim in low-Reynolds-number flows, revealing combined effects of rigid body rotation and chiral reorientation, with flow rate influencing their trajectories and orientation behaviors.

Contribution

It provides new insights into the swimming dynamics of filamentous E. coli under flow, highlighting the roles of rigid body rotation, chiral reorientation, and flow-dependent behaviors.

Findings

Rigid body rotation dominates in bacterial swimming.

Flow constrains trajectories and induces rheotaxis.

Non-motile bacteria follow streamlines without orientation bias.

Abstract

When treated with antibiotics below the minimum inhibitory concentration, bacterial cell division turns off, but cell growth does not. Thus, rod-like bacteria, including E. coli, can elongate many times their length without increasing their width. The swimming of these filamentous bacteria through small channels may provide insights into how bacteria that survive antibiotic treatment can reach channel walls. Such swimming behaviors in settings like hospital tubing may signal precursors to adhesion, biofilm formation, and infection. Despite the importance of understanding the behavior of bacteria not killed by antibiotics, the swimming of filamentous bacteria in external flows has not received much attention. We study the swimming behavior of stressed, filamentous E. coli. In quiescence, highly elongated E. coli swim with a sinusoidal undulating motion, suggesting rigid body rotation of long, rigid, buckled cell bodies. In low-Re pressure-driven microchannel flow, the undulation becomes irregular; it may even stop and start within a particular trajectory. We refer to this behavior in flow as "wiggling". Rigid body rotation persists in flow, appearing as a high-frequency change in body orientation on top of a slower one that can be explained by chiral reorientation. We quantify swimming behaviors in two different flow rates and observe rheotaxis in addition to preferential orientation of bacterial bodies. Faster flow constrains wiggling bacteria trajectories and orientations compared to those observed in slower flow, with rheotaxis taking bacteria toward the wall. But not all bacteria in flow wiggle. Populations of non-motile "non-wiggling" filamentous E. coli follow streamlines, without preferential orientation of their bodies. Non-motile bacteria do not behave like chiral rods propelled by rotating flagellar bundles, but like rigid rods. Motility slows swimmers in comparison.