Characterisation of the signal to noise ratio of 2-photon microscopes
Radek Mach\'a\v{n}, Shau Poh Chong, Khee Leong Lee, Peter T\"or\"ok

TL;DR
This paper characterizes the signal-to-noise ratio of a custom 2-photon microscope and compares it with commercial models, providing a methodology for benchmarking similar imaging systems.
Contribution
It introduces a methodology for characterizing and benchmarking the SNR of 2-photon microscopes, applicable to custom and commercial systems.
Findings
Custom microscope's SNR performance evaluated
Comparison with commercial microscopes conducted
Methodology can guide future SNR benchmarking
Abstract
Signal to noise ratio (SNR) is a key performance metric of an imaging instrument. Here we describe characterisation of SNR of a custom 2-photon microscope and compare the SNR performance of our microscope with selected commercial 2-photon microscopes. The methodology described in this paper can serve as guidance for others wishing to characterise and benchmark their 2-photon or other point-scanning microscopes.
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Taxonomy
TopicsAdvanced Fluorescence Microscopy Techniques · Image Processing Techniques and Applications · Integrated Circuits and Semiconductor Failure Analysis
