Curvature-adaptive gigapixel microscopy at submicron resolution and centimeter scale

TL;DR

PANORAMA is a novel gigapixel microscopy system that captures large-area, high-resolution images in a single shot without mechanical scanning, effectively imaging flat and curved samples over centimeter scales.

Contribution

It introduces a curvature-adaptive, single-shot gigapixel microscopy technique that maintains submicron focus across uneven samples, enhancing throughput and versatility.

Findings

Achieves 0.84 μm resolution over 16.3×18.8 mm² FOV in one shot

Maintains focus on curved and uneven samples across centimeters

Eliminates the need for mechanical scanning in large-area microscopy

Abstract

Large-area microscopy with submicron resolution is limited by tradeoffs between field of view (FOV), resolution, and imaging speed. Samples are rarely flat across centimeter-scale FOV, which often requires existing solutions to use mechanical scanning to ensure focused capture at reduced throughput. Here, we present PANORAMA, a single-shot, re-imaging microscope that achieves seamless, gigapixel imaging over a 16.3$\times$18.8 $\text{mm}^2$ FOV at 0.84 um resolution without mechanical scanning. By using a telecentric photolithography lens, a large-aperture tube lens, and a flat micro-camera array with adaptive per-camera focus control, PANORAMA maintains submicron focus across flat, curved or uneven samples that span centimeters. This approach improves imaging throughput and adaptability, enabling gigapixel multi-modal microscopy of large flat and non-flat samples in one shot, thus broadening its applications in biomedical and materials imaging.