Gliding microtubules exhibit tunable collective rotation driven by chiral active forces

TL;DR

This study reveals how chiral active forces at the microscopic level drive tunable collective rotation in microtubule systems, advancing understanding of chirality propagation in active matter.

Contribution

It introduces a new mechanism where motor-induced chiral forces cause collective rotation, independent of filament shape chirality, and shows how filament stiffness modulates this behavior.

Findings

Chiral active forces induce collective rotation in microtubule assays.

Simulation predicts rotation speed and handedness can be tuned by filament stiffness.

Chirality propagates from molecular forces to large-scale collective motion.

Abstract

How chirality propagates across scales remains an open question in many biological and synthetic systems. An especially clear manifestation of this propagation is found in in vitro gliding assays of cytoskeletal filaments on surfaces, driven by molecular motors. These assays have become model systems of active matter dynamics, as they spontaneously organize into diverse dynamical states, including collective motions with chiral rotation. However, the microscopic mechanisms underlying these chiral collective dynamics have remained unclear. Here, we investigate rotating active nematic order in microtubule gliding assay experiments under two stabilization conditions, each on two types of substrates. We propose that chirality in active forces exerted by motors on microtubules represents a viable mechanism for this large-scale chirality. Using Brownian dynamics simulations of self-propelled, semiflexible filaments with chiral activity, we demonstrate that coherently rotating active nematic order emerges by this mechanism even in the absence of curvature, i.e. shape chirality, of the constituent filaments. Moreover, we predict that the angular speed and handedness of the collective rotation can be tuned by modulating filament stiffness. Our findings identify a new set of sufficient microscopic ingredients for predictable propagation of chiral handedness from the molecular to the material scale in living and active matter.