Rotational Dynamics of ATP Synthase: Mechanical Constraints and Energy Dissipative Channels

TL;DR

This paper analyzes the energy transduction and dissipation in ATP synthase, quantifies internal friction as the main loss, and examines quantum mechanical constraints, concluding the enzyme operates within a classical regime optimized for efficiency.

Contribution

It provides a detailed energy accounting of ATP synthase, quantifies dissipation channels, and assesses quantum limits on rotation, revealing classical operation despite nanoscale size.

Findings

Internal friction is the dominant dissipation channel.

Biological rotation speeds are within quantum limits.

ATP synthase operates in a classical regime despite nanoscale dimensions.

Abstract

The proton motive force (PMF) across the inner mitochondrial membrane delivers approximately 0.2 eV of energy per proton, powering the FoF1-ATP synthase molecular motor. Here, we provide a detailed accounting of how this energy is utilized: Approximately 75-83% is transduced into the chemical free energy of ATP synthesis, while the remaining 17-25% is dissipated through internal friction, viscous drag, proton leakage, electroviscous effects, elastic deformations, and information-theoretic costs. Each dissipation channel is quantitatively evaluated, revealing that internal friction in the F1 motor is the dominant loss mechanism. In this work, we did not account for the energy supplied/injected due to the intrinsic electrostatic potential of the enzyme itself. In addition to this energy bookkeeping, we also examine the quantum mechanical constraints on the Fo unit's rotation. We find that, as can be expected, the energy spacing between quantized rotational states is several orders of magnitude smaller than thermal energies at physiological temperature, and that the tunneling probability through rotational barriers practically totally non-existent. Furthermore, the biological rotation speed (100-650 revolutions per second (rps)) is between one and three orders of magnitude below the quantum limit implied by quantization of angular momentum of the c-ring (which would have been ca. 13,000 to 62,000 rps (depending on the size of the c-ring (17 to 8 subunits, respectively)) in the first rotational energy level of the c-ring). Nevertheless, experimental estimates of the rotation rates in isolated c-ring suggest rates in the vicinity of 43,000 rps, right within our theoretical quantum estimates. However, ATP synthase as a whole operates firmly within the classical regime, despite its nanoscale dimensions, and highlight its evolutionary optimization for robust and efficient energy conversion....