Inline calibration of spatial light modulators in nonlinear microscopy
Dani\"el W.S. Cox, Harish Sasikumar, Ivo M. Vellekoop
TL;DR
This paper introduces a calibration method for phase-only spatial light modulators in nonlinear microscopy that uses the microscope image for calibration, eliminating extra hardware and functioning effectively under challenging conditions.
Contribution
A novel calibration approach leveraging the microscope image itself, tailored for nonlinear microscopy, especially multi-photon excitation, without additional hardware.
Findings
Effective calibration under low light conditions
Robust performance despite photobleaching
No extra hardware required
Abstract
We present a method for calibrating the response of a phase-only spatial light modulator in nonlinear microscopy. Our method uses the microscope image itself as calibration measurement and requires no additional hardware components. Our method is adapted to the nonlinear signals encountered in multi-photon excitation fluorescence microscopes, and works well even under low light conditions and with strong photobleaching.
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