Determining the utility of ultrafast nonlinear contrast enhanced and super resolution ultrasound for imaging microcirculation in the human small intestine

TL;DR

This study demonstrates the first application of ultrafast contrast-enhanced and super-resolution ultrasound imaging to visualize and quantify microcirculation in the human small intestine, revealing changes in blood flow after nutrient intake.

Contribution

It introduces non-invasive ultrafast CEUS and SRUS/ULM techniques for high-resolution imaging of intestinal microcirculation in vivo, with quantification of flow dynamics.

Findings

Ultrafast CEUS and SRUS/ULM successfully imaged human small intestine microcirculation.

Microvascular perfusion increased significantly after nutrient ingestion.

Flow speed within intestinal microvessels was quantified at approximately 2.78 mm/s.

Abstract

The regulation of intestinal blood flow is critical to gastrointestinal function. Imaging the intestinal mucosal micro-circulation in vivo has the potential to provide new insight into the gut physiology and pathophysiology. We aimed to determine whether ultrafast contrast enhanced ultrasound (CEUS) and super-resolution ultrasound localisation microscopy (SRUS/ULM) could be a useful tool for imaging the small intestine microcirculation in vivo non-invasively and for detecting changes in blood flow in the duodenum. Ultrafast CEUS and SRUS/ULM were used to image the small intestinal microcirculation in a cohort of 20 healthy volunteers (BMI<25). Participants were imaged while conscious and either having been fasted, or following ingestion of a liquid meal or water control, or under acute stress. For the first time we have performed ultrafast CEUS and ULM on the human small intestine, providing unprecedented resolution images of the intestinal microcirculation. We evaluated flow speed inside small vessels in healthy volunteers (2.78 +/- 0.05 mm/s, mean +/- SEM) and quantified changes in the perfusion of this microcirculation in response to nutrient ingestion. Perfusion of the microvasculature of the intestinal mucosa significantly increased post-prandially (36.2% +/- 12.2%, mean +/- SEM, p<0.05). The feasibility of 3D SRUS/ULM was also demonstrated. This study demonstrates the potential utility of ultrafast CEUS for assessing perfusion and detecting changes in blood flow in the duodenum. SRUS/ULM also proved a useful tool to image the microvascular blood flow in vivo non-invasively and to evaluate blood speed inside the microvasculature of the human small intestine.