Pan-genome Analysis of Plastomes from Lamiales using PGR-TK

TL;DR

This study used PGR-TK to analyze chloroplast genomes across Lamiales, revealing high sequence uniformity overall but notable divergence in specific genera and families, and effectively distinguishing and classifying plant groups.

Contribution

Introduces PGR-TK for pan-genome analysis of Lamiales plastomes, demonstrating its effectiveness in identifying divergence and classification accuracy.

Findings

High sequence uniformity in most genera and families

Utricularia, Incarvillea, and Orobanche are highly divergent

PGR-TK accurately distinguished genera and identified misclassifications

Abstract

Chloroplast sequences from the Lamiales order were analyzed using the Pangenome Research Toolkit (PGR-TK). Overall, most genera and families exhibited a high degree of sequence uniformity. However, at the genus level, Utricularia, Incarvillea, and Orobanche stood out as particularly divergent. At the family level, Orobanchaceae, Bignoniaceae and Lentibulariaceae displayed notably complex patterns in the generated plots. The PGR-TK algorithm successfully distinguished most genera within their respective families and often recognized misclassified plants.