ADNP-15: An Open-Source Histopathological Dataset for Neuritic Plaque Segmentation in Human Brain Whole Slide Images with Frequency Domain Image Enhancement for Stain Normalization

TL;DR

This paper introduces ADNP-15, an open-source histopathological dataset for neuritic plaque segmentation in brain images, and proposes a novel image enhancement method to improve deep learning model performance amidst staining variations.

Contribution

The study provides a large annotated dataset, evaluates multiple models and normalization techniques, and introduces a new image enhancement approach for better plaque segmentation.

Findings

Enhancement method improves segmentation accuracy significantly.

Stain normalization techniques influence model performance.

Open-source dataset facilitates further research in AD pathology.

Abstract

Alzheimer's Disease (AD) is a neurodegenerative disorder characterized by amyloid-beta plaques and tau neurofibrillary tangles, which serve as key histopathological features. The identification and segmentation of these lesions are crucial for understanding AD progression but remain challenging due to the lack of large-scale annotated datasets and the impact of staining variations on automated image analysis. Deep learning has emerged as a powerful tool for pathology image segmentation; however, model performance is significantly influenced by variations in staining characteristics, necessitating effective stain normalization and enhancement techniques. In this study, we address these challenges by introducing an open-source dataset (ADNP-15) of neuritic plaques (i.e., amyloid deposits combined with a crown of dystrophic tau-positive neurites) in human brain whole slide images. We establish a comprehensive benchmark by evaluating five widely adopted deep learning models across four stain normalization techniques, providing deeper insights into their influence on neuritic plaque segmentation. Additionally, we propose a novel image enhancement method that improves segmentation accuracy, particularly in complex tissue structures, by enhancing structural details and mitigating staining inconsistencies. Our experimental results demonstrate that this enhancement strategy significantly boosts model generalization and segmentation accuracy. All datasets and code are open-source, ensuring transparency and reproducibility while enabling further advancements in the field.