Clot Treatment via Compression- and Shear-Induced Densification of Fibrin Network Microstructure: A Combined in Vitro and In Silico Investigation

TL;DR

This study combines experiments and simulations to understand how compression and shear forces densify fibrin networks in blood clots, aiming to improve thrombectomy device design.

Contribution

It introduces an integrated in vitro and in silico approach to analyze clot microstructure changes under mechanical forces, advancing thrombectomy technology.

Findings

Clot volume can be reduced by up to 95% through combined compression and shear.

Simulations reveal fibrin network densification and RBC release mechanisms.

Microstructural insights guide the design of improved thrombectomy devices.

Abstract

Blood clots, consisting of red blood cells (RBCs) entrapped within a fibrin network, can cause life-threatening conditions such as stroke and heart attack. The recently developed milli-spinner thrombectomy device presents a promising mechanical approach to removing clots by substantially modifying the microstructure of the blood clot, resulting in up to 95% volume reduction through combined compressive and shear forces. To better understand the mechanism and optimize this approach, it is important to quantitatively understand of how compression and shear loadings alter the clot structure. In this study, we combine in vitro experiments with dissipative particle dynamics (DPD) simulations to investigate the effectiveness of clot debulking under integrated compression and shear. Controlled experiments quantify clot volume changes, while simulations offer microscopic insight into fibrin network densification and RBC release. This integrated approach enables a systematic evaluation of mechanical response and microstructure change of different clot types, providing fundamental knowledge to guide the rational design of next-generation mechanical thrombectomy technologies.