Seeing the unseen: laser speckles as a tool for coagulation tracking

TL;DR

This paper introduces a simple laser speckle rheology technique to monitor protein gelation in milk, offering a rapid, contactless, and cost-effective alternative to traditional rheological methods, with results aligning well with conventional measurements.

Contribution

The work presents a novel laser speckle rheology setup for tracking protein coagulation, demonstrating its effectiveness and potential as a quick, non-contact alternative to standard methods.

Findings

Laser speckle rheology accurately tracks coagulation and gelation times.

The viscoelasticity index correlates with complex modulus in similar samples.

The method is cost-effective and suitable for rapid protein gelation monitoring.

Abstract

The ability to measure protein functionality is critical for the development of plant-based products, particularly with respect to gelation behavior, which is vital for food structure and texture. Small amplitude oscillatory shear tests remain the standard for monitoring protein gelation; however, these methods are costly, time-consuming, and require physical contact with the sample. Laser speckle rheology, an optical-based technique, offers a contactless alternative by assessing rheological properties through speckle pattern fluctuations. In this work, we present a simple laser speckle rheology setup, utilizing a diode laser and a digital camera, to monitor rheological changes during the rennet coagulation of milk. We use a viscoelasticity index, derived from a two-dimensional linear correlation, to quantify speckle pattern fluctuations. The laser speckle rheology method is compared with conventional small amplitude oscillatory shear rheology. Results demonstrate that key characteristics of the coagulation process, including coagulation and gelation times, are temporally aligned between the two methods. Furthermore, the viscoelasticity index allows for the comparison of the complex modulus in samples with similar compositions under consistent acquisition parameters. These findings underscore the potential of laser speckle rheology as a cost-effective, rapid, and contactless approach for capturing protein gelation, providing a viable alternative to conventional shear rheological methods.