Cytogenetic, Hematobiochemical, and Histopathological Assessment of Albino Rats (Rattus norvegicus) Fed on Gluten Extracts

TL;DR

This study evaluated gluten extract toxicity in albino rats through various biological assessments, finding no adverse effects but noting some hematobiochemical alterations within normal ranges.

Contribution

It provides comprehensive in vivo toxicological data on gluten, addressing previous inconsistencies and supporting gluten's safety at tested doses.

Findings

No significant impact on body weight or blood glucose.

Hematobiochemical parameters altered but within normal limits.

No histopathological damage to liver and kidneys.

Abstract

Background: Literature shows that most of the information on the toxicity of gluten is generated from survey and observational studies, resulting in inconsistent outcomes and a decrease in the acceptability of gluten-rich foods. To determine gluten's safety, an in-depth in vitro and in vivo toxicological examination is required. This enables scientists to come up with ameliorative strategies if it turns out to have side effects, and consumers' trust can be restored. Objectives: The objective of this study was to assess the toxicity of gluten extracts on albino rats (Rattus norvegicus). Materials and Methods: Twenty-four rats were randomly selected and divided into four groups, each comprising six rats. Group 1 (control) rats were fed on pellet feeds and groups 2, 3, and 4 were fed on daily dosages of 0.5, 1.0, and 1.5 g gluten extracts, respectively. The rats' body weights and reactions were observed for 90 days before blood samples were collected for hematobiochemical and micronucleus tests. Histopathological examinations of the liver and kidneys were also performed. Results: There was no difference (P > 0.05) in body weight, blood glucose level, or micronuclei between the control and treated rats. The lymphocytes, alkaline phosphatase, alanine transaminase, total protein, and calcium ions of the test rats were all significantly (P < 0.05) altered but remained within the normal ranges. Other hematobiochemical parameters, including packed cell volume, hemoglobin, white and red blood cells, aspartate transaminase, albumin, sodium ions, potassium ions, chloride ions, and urea, revealed no marked changes. The treated rats' livers and kidneys showed no histopathological changes. Conclusion: Gluten had no adverse effects. However, it altered hematobiochemical parameters, particularly the lymphocytes, alkaline phosphatase, alanine transaminase, total protein, and calcium ions.