Spatial-offset pump-probe imaging of nonradiative dynamics at optical resolution

TL;DR

The paper introduces SOPPI, a high-resolution imaging technique that maps nonradiative wave dynamics with nanosecond and micrometer precision, advancing biological imaging and understanding of photothermal and photoacoustic processes.

Contribution

SOPPI is a novel spatial offset pump-probe imaging method that enables simultaneous, high-resolution mapping of PA and PT wave propagation in biological tissues.

Findings

Successfully mapped PA and PT wave evolution from fiber emitters.

Demonstrated wavelength-dependent PA generation and evanescent wave effects.

Reconstructed pigment distribution in zebrafish with high precision.

Abstract

Nonradiative photothermal (PT) and photoacoustic (PA) processes have found widespread applications in imaging, stimulation, and therapy. Mapping the generation and propagation of PA and PT waves with resolution is important to elucidate how these fields interact with biological systems. To this end, we introduce spatial offset pump-probe imaging (SOPPI). By spatially offsetting the pump beam and the probe beam, SOPPI can image simultaneously PA and PT wave propagation with nanosecond temporal resolution, micrometer spatial resolution, 65 MHz detection bandwidth, and a sensitivity of 9.9 Pa noise equivalent pressure. We first map the PA and PT evolution from a fiber emitter, and how the wave interacting with a mouse skull and brain slices. SOPPI imaging of PA waves from a tapered fiber with water as an absorber shows a wavelength-dependent generation, evanescent wave generated PA, and back-propagated acoustic Mach Cone. At last, a SOPPI-PACT is developed to reconstruct the pigment distribution inside a zebrafish larva with high precision and signal-to-noise ratio.