Sensitive bioassay with an ultra-large dynamic range via microlaser ensemble quenching

TL;DR

This paper introduces a microlaser ensemble bioassay that uses lasing threshold distribution to detect analyte concentrations with ultra-large dynamic range and high sensitivity, suitable for various biomolecular detection applications.

Contribution

The study presents a novel microlaser ensemble platform that maps lasing threshold distributions to analyte concentrations, achieving high sensitivity and broad dynamic range in bioassays.

Findings

Detection limit of 0.1 pg/mL for analytes.

Dynamic range exceeds five orders of magnitude.

Effective for biomolecules like streptavidin and interleukin-6.

Abstract

We present a bioassay platform that leverages the lasing threshold distribution in a microlaser ensemble (ME), consisting of hundreds of individual microlasers, to measure analyte concentrations in solution. An ME is formed by placing dye-doped microbeads in a micro Fabry-Perot cavity. Microbeads are surface modified with biorecognition molecules to capture analytes, while the quenchers resulting from the presence of the analytes on the microbeads' surfaces increase the lasing thresholds of microlasers. Since the number of analytes varies from one microbead (or microlaser) to another due to the randomness in binding processes, a distribution of the analytes (and hence the quenchers) in the ME is created, which in turn leads to a lasing threshold distribution in the ME. Experimentally, multiple pumping energy densities are used to probe the lasing threshold distribution. A theoretical model is developed to map the lasing threshold distribution to analyte distribution in the ME, and then to recover the analyte concentration in solution. Using streptavidin and interleukin-6 as a model system, our platform achieves a detection limit of 0.1 pg/mL and a dynamic range exceeding five orders of magnitude, showing that the ME quenching method can provide a high sensitivity with a superior dynamic range.