Fast Wide-field Light Sheet Electro-optic FLIM

TL;DR

This paper presents a fast, wide-field volumetric fluorescence lifetime imaging microscopy (FLIM) method using electro-optic gating with a Pockels cell, achieving large field of view and live sample imaging.

Contribution

It introduces a novel electro-optic FLIM technique integrated with light sheet microscopy for rapid 3D fluorescence lifetime imaging.

Findings

Achieved up to 800 μm field of view in light sheet FLIM.

Successfully imaged fluorescent beads and live plant samples.

Demonstrated compatibility with genetically encoded and autofluorescent signals.

Abstract

We demonstrate volumetric fluorescence lifetime microscopy (FLIM) using the electro-optic FLIM technique. Images acquired in a selective plane illumination microscope are gated using a Pockels cell driven at 80 MHz, enabling light sheet FLIM acquisition with up to 800 {\mu}m field of view. Volume acquisitions are demonstrated on fluorescent bead mixtures and in live Arabidopsis thaliana root samples using both genetically encoded fluorescent proteins and endogenous autofluorescence.