In Vivo Study of Bone Growth Around Additively Manufactured Implants with Ti-6Al-4V and Bioactive Glass Powder Composites

TL;DR

This study investigates how bioactive glass enhances bone integration around additively manufactured Ti-6Al-4V implants, comparing coating and doping methods through in vivo animal tests over 12 weeks.

Contribution

It systematically compares BG coating and doping in 3D-printed implants, revealing their effects on early and late-stage osseointegration.

Findings

BG accelerates early bone ingrowth

BG coating shows greater early-stage improvement

Effects diminish at 12 weeks

Abstract

Osseointegration is crucial to the success of biomedical implants. Additive manufacturing of implants offers a high degree of design freedom, enabling precise control over implant geometry and material composition. Bioactive glass (BG) can substantially enhance bone binding and bioactivity; however, limited research has been conducted on its incorporation into additively manufactured implants. The performance of BG varies depending on the incorporation method, and the spatial and temporal evolution of its integration remains unclear. In this study, we synthesized Ti-6Al-4V/58S BG composites by using the selective laser melting method and systematically compared the effects of BG coating and doping in additively manufactured implants. In vivo histological results from animal tests were statistically analyzed and discussed in terms of osseointegration over 4- and 12-week periods. Bone-to-implant contact (BIC) and bone density (BD) were used as quantitative metrics to evaluate interactions between the implants and surrounding bone. Our findings indicate that both BG-doped and BG-coated implants accelerated bone ingrowth during the early stages of healing. BG-coated implants demonstrated a greater improvement than did pure 3D-printed Ti-6Al-4V implants. However, the effects of BG became nonsignificant during the later healing stage (12 weeks). This study provides a foundation for systematically investigating BG incorporation methods in 3D-printed biomedical implants and their effect on osseointegration.