Elucidating the high compliance mechanism by which the urinary bladder fills under low pressures

TL;DR

This study uncovers how large-scale folds in the bladder's mucosa enable high compliance during filling, using advanced imaging to reveal physical mechanisms that support bladder function and inform pathological understanding.

Contribution

It demonstrates that large mucosal folds, rather than small rugae, are responsible for high compliance in bladder filling, supported by detailed 3D imaging and pressure-volume analysis.

Findings

Large mucosal folds flatten to increase volume during filling.

Bladder achieves over 97% voiding efficiency.

Large-scale folds facilitate urine flow towards the outlet.

Abstract

The high compliance of the urinary bladder during filling is essential for its proper function, enabling it to accommodate significant volumetric increases with minimal rise in transmural pressure. This study aimed to elucidate the physical mechanisms underlying this phenomenon by analyzing the ex vivo filling process in rat from a fully voided state to complete distension, without preconditioning, using three complementary imaging modalities. High-resolution micro-CT at 10.8 {\mu}m resolution was used to generate detailed 3D reconstructions of the bladder lumen, revealing a 62 fold increase in bladder volume during filling. Pressure-volume studies of whole bladder delineated three mechanical filling regimes: an initial high-compliance phase, a transitional phase, and a final high-pressure phase. While prior studies conjectured small mucosal rugae (450 {\mu}m) are responsible for the high compliance phase, multiphoton microscopy (MPM) of the dome of the voided bladder revealed large folds an order of magnitude larger than these rugae. Bladder imaging during the inflation process demonstrated flattening of these large scale folds is responsible for volume increases in the initial high compliance phase. The 3D reconstructions of the bladder lumen in the filled and voided state revealed a high voiding efficiency of 97.13%. The MPM imaging results suggest the large scale folds in the dome enable this high voiding fraction by driving urine toward the bladder outlet. These insights are vital for computational models of bladder biomechanics and understanding changes to bladder function due to pathological conditions such as bladder outlet obstruction and age-related dysfunction.