Variable zoom digital in-line holographic microscopy

TL;DR

This paper introduces a variable zoom digital in-line holographic microscopy system utilizing an electrically tunable lens, allowing adjustable magnification and field of view without mechanical adjustments, validated on resolution targets and biological samples.

Contribution

The novel VZ-DIHM setup enables variable magnification using an electrically tunable lens, eliminating mechanical movements and enhancing biomedical imaging capabilities.

Findings

Achieves magnification from 15X to 35X

Validates performance with resolution test target

Demonstrates application on prostate cancer cells

Abstract

We report on a novel layout providing variable zoom in digital in-line holographic microscopy (VZ-DIHM). The implementation is in virtue of an electrically tunable lens (ETL) which enables to slightly shift the illumination source axial position without mechanical movement of any system component. Magnifications ranging from 15X to 35X are easily achievable using the same layout and resulting in a substantial variation of the total field of view (FOV). The performance of the proposed setup is, first, validated using a resolution test target where the main parameters are analyzed (theoretically and experimentally) and, second, corroborated analyzing biological sample (prostate cancer cells) showing its application to biomedical imaging.