Benchmarking of Fluorescence Lifetime Measurements using Time-Frequency Correlated Photons

TL;DR

This paper benchmarks a novel quantum-based fluorescence lifetime measurement method using entangled photons, demonstrating its advantages over traditional techniques and exploring its potential for quantum advantages in biological imaging.

Contribution

It introduces a new fluorescence lifetime measurement approach utilizing entangled photons and compares its performance with existing methods, highlighting potential quantum benefits.

Findings

Demonstrated fluorescence lifetime measurement of IR-140 using entangled photons

Compared the new method with state-of-the-art FLIM and observed improved figure-of-merit

Discussed potential quantum advantages in fluorescence lifetime imaging

Abstract

The investigation of fluorescence lifetime became an important tool in biology and medical science. So far, established methods of fluorescence lifetime measurements require the illumination of the investigated probes with pulsed or amplitude-modulated light. In this paper, we examine the limitations of an innovative method of fluorescence lifetime using the strong time-frequency correlation of entangled photons generated by a continuous-wave source. For this purpose, we investigate the lifetime of IR-140 to demonstrate the functional principle and its dependencies on different experimental parameters. We also compare this technique with state-of-the-art FLIM and observed an improved figure-of-merit. Finally, we discuss the potential of a quantum advantage.