Traction and Stress Control Formation and Motion of +1/2 Topological Defects in Epithelial Cell Monolayers

TL;DR

This study investigates how +1/2 topological defects form and move in epithelial cell monolayers, revealing that coordinated force and motion patterns precede defect formation and influence defect dynamics.

Contribution

It provides new insights into the formation and motion of +1/2 defects, emphasizing the role of pre-existing force and motion patterns in defect dynamics.

Findings

Both traction and stress drive defect motion.

Patterns of force and motion exist before defect formation.

Defects form from coordinated force and motion patterns.

Abstract

In confluent cell monolayers, patterns of cell forces and motion are systematically altered near topological defects in cell shape. In turn, defects have been proposed to alter cell density, extrusion, and invasion, but it remains unclear how the defects form and how they affect cell forces and motion. Here, we studied +1/2 defects, and, in contrast to prior studies, we observed the concurrent occurrence of both tail-to-head and head-to-tail defect motion in the same cell monolayer. We quantified the cell velocities, the tractions at the cell-substrate interface, and the stresses within the cell layer near +1/2 defects. Results revealed that both traction and stress are sources of activity and dissipation within the epithelial cell monolayer, with the direction of motion of +1/2 defects depending on whether energy is injected by stresses or tractions. Interestingly, patterns of motion, traction, stress, and energy injection near +1/2 defects existed before defect formation, suggesting that defects form as a result of spatially coordinated patterns in cell forces and motion. These findings introduce a new focus, on coordinated patterns of force and motion that lead to defect formation and motion.