The same but different: impact of animal facility sanitary status on a transgenic mouse model of Alzheimer's disease

TL;DR

This study demonstrates that the sanitary status of animal facilities significantly influences gut microbiota composition and Alzheimer's disease pathology in transgenic mice, affecting experimental outcomes and interpretations.

Contribution

It reveals the impact of housing conditions on microbiota and disease phenotypes, highlighting the importance of facility sanitation in preclinical Alzheimer's research.

Findings

Conventional housing increases amyloid pathology in 5XFAD mice.

Microbiota transplantation from different housing conditions affects memory performance.

Housing in SOPF conditions mitigates microbiota-related disease progression.

Abstract

The gut-brain axis has emerged as a key player in the regulation of brain function and cognitive health. Gut microbiota dysbiosis has been observed in preclinical models of Alzheimer's disease and patients. Manipulating the composition of the gut microbiota enhances or delays neuropathology and cognitive deficits in mouse models. Accordingly, the health status of the animal facility may strongly influence these outcomes. In the present study, we longitudinally analysed the faecal microbiota composition and amyloid pathology of 5XFAD mice housed in a specific opportunistic pathogen-free (SOPF) and a conventional facility. The composition of the microbiota of 5XFAD mice after aging in conventional facility showed marked differences compared to WT littermates that were not observed when the mice were bred in SOPF facility. The development of amyloid pathology was also enhanced by conventional housing. We then transplanted faecal microbiota (FMT) from both sources into wild-type (WT) mice and measured memory performance, assessed in the novel object recognition test, in transplanted animals. Mice transplanted with microbiota from conventionally bred 5XFAD mice showed impaired memory performance, whereas FMT from mice housed in SOPF facility did not induce memory deficits in transplanted mice. Finally, 18 weeks of housing SOPF-born animals in a conventional facility resulted in the reappearance of specific microbiota compositions in 5XFAD vs WT mice. In conclusion, these results show a strong impact of housing conditions on microbiota-associated phenotypes and question the relevance of breeding preclinical models in specific pathogen-free (SPF) facilities.