Extended electrochemical monitoring of biomolecular binding using commercially available, reusable electrodes in microliter volumes

TL;DR

This paper presents adapted electrochemical biosensing methods using commercially available, reusable electrodes suitable for novice users, enabling extended, low-volume biomolecular monitoring with simplified data analysis.

Contribution

It introduces user-friendly, low-cost electrochemical biosensing techniques with extended monitoring capabilities tailored for educational and beginner settings.

Findings

Compatible with reusable electrodes

Operates effectively in microliter volumes

Supports extended monitoring periods

Abstract

Electrochemical biosensors ("E-AB" or "E-DNA" type sensors) that utilize square-wave voltammetry originated in academic labs with a few standard experimental configurations for the electrochemical cell and data analysis. We report here on adaptations of these approaches that are friendly to novice scientists such as those in undergraduate laboratories. These approaches utilize commercially available components, low volumes, work over extended periods and enable facile analysis using a custom excel sheet.