Advances and Applications of Dynamic Surface-Enhanced Raman Spectroscopy (SERS) for Single Molecule Studies

TL;DR

This review discusses recent advancements in dynamic SERS techniques, emphasizing improved instrumentation, data analysis, and applications in single-molecule real-time studies of chemical and biological processes.

Contribution

It provides a comprehensive overview of the latest developments, applications, and challenges in dynamic SERS for single-molecule research.

Findings

Enhanced temporal resolution and sensitivity in dynamic SERS

Successful monitoring of chemical reactions and biomolecular interactions

Development of novel substrates for improved detection

Abstract

Dynamic surface-enhanced Raman spectroscopy (SERS) is nowadays one of the most interesting applications of SERS, in particular for single molecule studies. In fact, it enables the study of real-time processes at the molecular level. This review summarizes the latest developments in dynamic SERS techniques and their applications, focusing on new instrumentation, data analysis methods, temporal resolution and sensitivity improvements, and novel substrates. We highlight the progress and applications of single-molecule dynamic SERS in monitoring chemical reactions, catalysis, biomolecular interactions, conformational dynamics, and real-time sensing and detection. We aim to provide a comprehensive review on its advancements, applications as well as its current challenges and development frontiers.