Effect of Temperature and TiO$_{2}$ NP Concentration on DNA Stability under Conditions Close to Physiological Ones

TL;DR

This study investigates how temperature and TiO₂ nanoparticle concentration affect DNA stability near physiological conditions, revealing stable nanoassemblies and structural changes without aggregation at neutral pH.

Contribution

It provides new insights into DNA-TiO₂ nanoparticle interactions and stability under physiological-like conditions using thermal denaturation and DLS methods.

Findings

DNA forms ordered structures on TiO₂ NPs surface.

DNA-TiO₂ nanoassemblies remain stable across a wide temperature range.

No aggregation observed at pH 7, unlike at pH 5.

Abstract

The present work is devoted to the study of the thermostability of native DNA during binding to TiO$_{2}$ nanoparticles (NPs) at various concentrations under conditions close to physiological (0.1 M Na $^+$, pH 7) using thermal denaturation method and dynamic light scattering (DLS). The analysis of the DNA melting curves in the presence of TiO$_{2}$ NPs revealed a temperature range in which the light absorption of DNA decreases. This is explained by the formation of an ordered structure of partially unwound DNA strands on the NP surface. The DNA:TiO$_{2}$ NP nanoassemblies formed at the initial stages remained stable in the wide temperature range. The performed temperature-dependent DLS measurements of the DNA:TiO$_{2}$ NP suspension at pH 7 have not revealed the aggregation of the DNA:TiO$_{2}$ NP nanoassemblies that had been observed at pH 5.