Increasing the spatial bandwidth product in Light Field Microscopy with remote scanning

TL;DR

This paper introduces an enhanced light field microscopy technique with remote scanning, enabling faster, larger-scale volumetric imaging with improved resolution and field of view, suitable for biological applications.

Contribution

The authors present a simple, versatile augmented light field microscopy method using a motorized tilting mirror to improve imaging speed, resolution, and field of view.

Findings

Enhanced volumetric imaging with improved spatial bandwidth

Versatile imaging in bright-field and epifluorescence modes

Successful imaging of multi-cellular aggregates

Abstract

Achieving fast, large-scale volumetric imaging with micrometer resolution has been a persistent challenge in the field of biological microscopy. To address this challenge, we report an augmented version of light field microscopy, incorporating a motorized tilting mirror upstream the camera. Depending on the scanning pattern, the field of view and/or the lateral resolution can be greatly improved. Our microscope technique is simple, versatile and configured for both bright-field and epifluorescence modes. We demonstrate its performances with imaging of multi-cellular aggregates of various shape and sizes.