Retinal arterial blood flow measured by real-time Doppler holography at 33,000 frames per second

TL;DR

This paper introduces a high-speed Doppler holography technique at 33,000 fps for non-invasive, real-time measurement of retinal arterial blood flow, offering detailed insights into retinal health.

Contribution

It presents a novel quantitative method combining high-speed digital holography and Doppler analysis to measure retinal blood flow non-invasively.

Findings

Enables non-invasive retinal blood flow imaging.

Provides detailed blood velocity measurements.

Potential for improved diagnosis of retinal vascular diseases.

Abstract

This study presents a novel quantitative estimation method for total retinal arterial blood flow utilizing real-time Doppler holography at an unprecedented frame rate of 33,000 frames per second. This technique, leveraging high-speed digital holography, enables non-invasive angiographic imaging of the retina, providing detailed blood flow contrasts essential for assessing retinal health. The proposed quantitative analysis method consists of segmenting primary in-plane retinal arteries and calculating local blood velocity using Doppler frequency broadening. The analysis integrates a forward scattering model to achieve blood flow estimation. Our findings highlight the potential of Doppler holography as a powerful tool for diagnosing and monitoring the treatment of retinal vascular conditions, complementary to existing imaging methods.