Gene and RNA Editing: Methods, Enabling Technologies, Applications, and Future Directions

TL;DR

This paper reviews gene and RNA editing technologies, highlighting CRISPR and Cas13 as key tools, discussing their applications in medicine, disease detection, and future therapeutic directions.

Contribution

It provides a comprehensive overview of current editing methods, technologies, and applications, emphasizing recent advancements like CRISPR-Cas13 for RNA editing and future prospects.

Findings

CRISPR-Cas13 enables efficient RNA editing.

RNA editing offers temporary modifications unlike permanent DNA changes.

Advances in editing technologies enhance disease detection and therapeutic potential.

Abstract

Gene and RNA editing methods, technologies, and applications are emerging as innovative forms of therapy and medicine, offering more efficient implementation compared to traditional pharmaceutical treatments. Current trends emphasize the urgent need for advanced methods and technologies to detect public health threats, including diseases and viral agents. Gene and RNA editing techniques enhance the ability to identify, modify, and ameliorate the effects of genetic diseases, disorders, and disabilities. Viral detection and identification methods present numerous opportunities for enabling technologies, such as CRISPR, applicable to both RNA and gene editing through the use of specific Cas proteins. This article explores the distinctions and benefits of RNA and gene editing processes, emphasizing their contributions to the future of medical treatment. CRISPR technology, particularly its adaptation via the Cas13 protein for RNA editing, is a significant advancement in gene editing. The article will delve into RNA and gene editing methodologies, focusing on techniques that alter and modify genetic coding. A-to-I and C-to-U editing are currently the most predominant methods of RNA modification. CRISPR stands out as the most cost-effective and customizable technology for both RNA and gene editing. Unlike permanent changes induced by cutting an individual's DNA genetic code, RNA editing offers temporary modifications by altering nucleoside bases in RNA strands, which can then attach to DNA strands as temporary modifiers.