CHOTA: A Higher Order Accuracy Metric for Cell Tracking

TL;DR

The paper introduces CHOTA, a new cell tracking evaluation metric that assesses global coherence and lineage accuracy, addressing limitations of existing metrics and improving biological relevance in cell tracking analysis.

Contribution

We propose CHOTA, a comprehensive cell tracking metric that unifies detection, association, coherence, and lineage evaluation, enhancing biological insight.

Findings

CHOTA is sensitive to all tracking errors.

It provides a better indication of lineage reconstruction quality.

It outperforms existing metrics in biological relevance.

Abstract

The evaluation of cell tracking results steers the development of tracking methods, significantly impacting biomedical research. This is quantitatively achieved by means of evaluation metrics. Unfortunately, current metrics favor local correctness and weakly reward global coherence, impeding high-level biological analysis. To also foster global coherence, we propose the CHOTA metric (Cell-specific Higher Order Tracking Accuracy) which unifies the evaluation of all relevant aspects of cell tracking: cell detections and local associations, global coherence, and lineage tracking. We achieve this by introducing a new definition of the term 'trajectory' that includes the entire cell lineage and by including this into the well-established HOTA metric from general multiple object tracking. Furthermore, we provide a detailed survey of contemporary cell tracking metrics to compare our novel CHOTA metric and to show its advantages. All metrics are extensively evaluated on state-of-the-art real-data cell tracking results and synthetic results that simulate specific tracking errors. We show that CHOTA is sensitive to all tracking errors and gives a good indication of the biologically relevant capability of a method to reconstruct the full lineage of cells. It introduces a robust and comprehensive alternative to the currently used metrics in cell tracking. Python code is available at https://github.com/CellTrackingChallenge/py-ctcmetrics .