Harnessing quantum light for microscopic biomechanical imaging of cells and tissues

TL;DR

This paper introduces a quantum-light-enhanced stimulated Brillouin scattering imaging technique that significantly reduces photo-damage in live biological samples, enabling longer, non-destructive biomechanical imaging at the cellular level.

Contribution

The study presents a novel quantum-light-based approach to improve SBS imaging contrast, increasing sample viability and extending imaging duration in live tissues.

Findings

Three-fold increase in sample viability with squeezed-light illumination

Significant enhancement in signal-to-noise ratio for SBS imaging

Extended imaging times without damaging live samples

Abstract

The biomechanical properties of cells and tissues play an important role in our fundamental understanding of the structures and functions of biological systems at both the cellular and subcellular levels. Recently, Brillouin microscopy, which offers a label-free spectroscopic means of assessing viscoelastic properties in vivo, has emerged as a powerful way to interrogate those properties on a microscopic level in living tissues. However, susceptibility to photo-damage and photo-bleaching, particularly when high-intensity laser beams are used to induce Brillouin scattering, poses a significant challenge. This article introduces a transformative approach designed to mitigate photo-damage in biological and biomedical studies, enabling non-destructive, label-free assessments of mechanical properties in live biological samples. By leveraging quantum-light-enhanced stimulated Brillouin scattering (SBS) imaging contrast, the signal-to-noise ratio is significantly elevated, thereby increasing sample viability and extending interrogation times without compromising the integrity of living samples. The tangible impact of this novel methodology is evidenced by a notable three-fold increase in sample viability observed after subjecting the samples to three hours of continuous squeezed-light illumination, surpassing the traditional coherent light-based approaches. The quantum-enhanced SBS imaging holds promise across diverse fields, such as cancer biology and neuroscience where preserving sample vitality is of paramount significance. By mitigating concerns regarding photo-damage and photo-bleaching associated with high-intensity lasers, this technological breakthrough expands our horizons for exploring the mechanical properties of live biological systems, paving the way for a new era of research and clinical applications.