Thermal Preconditioning of Membrane Stress to Control the Shapes of Ultrathin Crystals

TL;DR

This study demonstrates how thermal preconditioning of membrane tension influences the shape and growth of ultrathin phospholipid crystals within vesicles, revealing a physical mechanism for controlling crystal morphology through membrane stress regulation.

Contribution

It introduces a novel method of controlling crystal shape by preconditioning membrane tension via cooling rate, supported by a physical model of stress evolution based on membrane properties.

Findings

Crystal shapes are regulated by membrane tension influenced by cooling rate.

Larger vesicles develop more elaborate crystal morphologies.

The model predicts tension behaviors consistent with experimental observations.

Abstract

We employ the phospholipid bilayer membranes of giant unilamellar vesicles as a free-standing environment for the growth of membrane-integrated ultrathin phospholipid crystals possessing a variety of shapes with 6-fold symmetry. Crystal growth within vesicle membranes, where more elaborate shapes grow on larger vesicles is dominated by the bending energy of the membrane itself, creating a means to manipulate crystal morphology. Here we demonstrate how cooling rate preconditions the membrane tension before nucleation, in turn regulating nucleation and growth, and directing the morphology of crystals by the time they are large enough to be visualized. The crystals retain their shapes during further growth through the two phase region. Experiments demonstrate this behavior for single crystals growing within the membrane of each vesicle, ultimately comprising up to 13% of the vesicle area and length scales of up to 50 microns. A model for stress evolution, employing only physical property data, reveals how the competition between thermal membrane contraction and water diffusion from tensed vesicles produces a size- and time-dependence of the membrane tension as a result of cooling history. The tension, critical in the contribution of bending energy in the fluid membrane regions, in turn selects for crystal shape for vesicles of a given size. The model reveals unanticipated behaviors including a low steady state tension on small vesicles that allows compact domains to develop, rapid tension development on large vesicles producing flower-shaped domains, and a stress relaxation through water diffusion across the membrane with a time constant scaling as the square of the vesicle radius, consistent with measurable tensions only in the largest vesicles.