Towards ultrasensitive biosensors based on virus-like particles and plasmonic surface lattice resonance

TL;DR

This paper demonstrates that organizing virus-like particles into plasmonic surface lattice resonance arrays significantly enhances vibrational signals, paving the way for ultrasensitive biosensors capable of detecting extremely low concentrations of antibodies.

Contribution

The study introduces a novel approach of using ordered VLP arrays exhibiting SLR to amplify signals for ultrasensitive biodetection, surpassing previous LSPR-based methods.

Findings

SLR arrays amplify signals by two orders of magnitude.

Ordered VLPs enable detection of low-concentration antibodies.

SLR-based biosensors show potential for ultrasensitive detection.

Abstract

Plasmonic surface lattice resonance (SLR) is a phenomenon in which individual localized surface plasmon resonances (LSPRs) excited in periodically-arranged plasmonic nanoparticles couple through the interaction with propagating diffracted incident light. The SLR optical absorption peak is by at least one order of magnitude more intense than the LSPR one, making SLR superior for applications in which LSPR is commonly used. Recently, we have developed a route for the fabrication of spherical virus-like particles (VLPs) with plasmonic cores and protein coronas, where the LSPR in the cores amplifies vibrational signals originating from protein-antibody bonding, showing the potential of VLPs in biodetection. However, the signals were not strong enough to detect antibodies at very low concentrations. Here, we show that by ordering the VLPs in periodic nanoarrays exhibiting SLR amplifies the signals by two orders of magnitude, revealing superior potential of SLR arrays in ultrasensitive biodetection.