Mid-Infrared Hyperspectral Microscopy with Broadband 1-GHz Dual Frequency Combs

TL;DR

This paper demonstrates a compact, high-speed mid-infrared hyperspectral microscopy system using broadband 1-GHz dual-comb spectrometers, enabling detailed chemical imaging of biological tissues and polymers with high resolution and spectral coverage.

Contribution

The study integrates a broadband, high-repetition-rate dual-comb spectrometer with a scanning microscope, showcasing high-speed, broadband hyperspectral imaging in the mid-infrared range.

Findings

Achieved 5 μm spatial resolution in hyperspectral imaging.

Demonstrated 12.86 kHz spectral acquisition rate.

Covered 1000 cm^{-1} bandwidth at 2941 cm^{-1} center frequency.

Abstract

Mid-infrared microscopy is an important tool for biological analyses, allowing a direct probe of molecular bonds in their low energy landscape. In addition to the label-free extraction of spectroscopic information, the application of broadband sources can provide a third dimension of chemical specificity. However, to enable widespread deployment, mid-infrared microscopy platforms need to be compact and robust while offering high speed, broad bandwidth and high signal-to-noise ratio (SNR). In this study, we experimentally showcase the integration of a broadband, high-repetition-rate dual-comb spectrometer (DCS) in the mid-infrared range with a scanning microscope. We employ a set of 1-GHz mid-infrared frequency combs, demonstrating their capability for high-speed and broadband hyperspectral imaging of polymers and ovarian tissue. The system covers 1000 $\mathrm{cm^{-1}}$ at $\mathrm{\nu_c=2941 \; cm^{-1}}$ with 12.86 kHz spectra acquisition rate and 5 $\mathrm{\mu m}$ spatial resolution. Taken together, our experiments and analysis elucidate the trade-off between bandwidth and speed in DCS as it relates to microscopy. This provides a roadmap for the future advancement and application of high-repetition-rate DCS hyperspectral imaging.