Rapid IRMPD analysis for glycomics

TL;DR

This paper demonstrates a rapid IRMPD technique for glycomics that significantly reduces acquisition time, enabling high-resolution molecular analysis with improved efficiency and potential for complex analytical workflows.

Contribution

It introduces an optimized combination of mass spectrometry and tunable laser to drastically enhance IRMPD performance for glycomics analysis.

Findings

Achieved two orders of magnitude improvement in IRMPD efficiency

Resolved spectra in less than one minute

Enabled new applications in glycomics workflows

Abstract

Infrared vibrational spectroscopy in the gas phase has emerged as a powerful tool to determine complex molecular structures with Angstrom accuracy. Among the different approaches IRMPD (InfraRed Multiple Photon Dissociation), which requires the use of an intense pulsed tunable laser in the IR domain, has been broadly applied to the study of complex (bio)molecules. Recently, it also emerged as a highly relevant approach for analytical purposes especially in the field of glycomics in which structural analysis is still a tremendous challenge. This opens the perspective to develop new analytical tools allowing for the determination of molecular structures with atomic precision, and to address advanced questions in the field. However, IRMPD experiments require either non commercial equipments and long acquisition time which limits the data output. Here we show that it is possible to improve the IRMPD performances by optimizing the combination between a LTQ XL mass spectrometer and a high repetition tunable laser Firefly. Two orders of magnitude are gained with this approach compared to usual experiments ultimately leading to a completely resolved spectrum acquired in less than one minute. These results open the way to many new applications in glycomics with the possibility to include IRMPD in complex analytical workflows.