Complementarity between Brillouin signature and Scholte wave for controlled elasticity in fibrillated collagen medium for culture cell

TL;DR

This paper combines Brillouin spectroscopy and Scholte wave techniques to precisely measure how fibrillar collagen affects ECM elasticity, advancing tissue engineering and cellular biomechanics understanding.

Contribution

It introduces a novel combined approach using Brillouin and Scholte wave methods to assess collagen fibrillation effects on ECM elasticity.

Findings

Fibrillation influences sound velocity and refractive index in collagen.

The combined method improves measurement precision of ECM elasticity.

Insights support advancements in tissue engineering and biomechanics.

Abstract

Modulating extracellular matrix (ECM) elasticity with fibrillar collagen offers great potential for regenerative medicine, drug discovery, and disease modeling by replicating invivo mechanical signals. This enhances understanding of cellular responses and fosters therapeutic innovation. However, precise ECM elasticity measurements are still lacking. This study couples time-resolved Brillouin spectroscopy and pulsed laser-induced Scholtes wave generation. We measure how collagen fibrillation affects sound velocity and refractive index. These insights are advancing tissue engineering and cellular biomechanics.