Diamond molecular balance: Revolutionizing high-resolution mass spectrometry from MDa to TDa at room temperature

TL;DR

The paper introduces a diamond-based molecular balance sensor capable of high-resolution mass spectrometry from megadaltons to teradaltons at room temperature, enabling precise single-particle mass measurements.

Contribution

It presents a novel diamond nanostructure sensor with exceptional mass resolution and dynamic range, advancing room-temperature mass spectrometry technology.

Findings

Achieved 0.36 MDa mass resolution

Extended dynamic range from MDa to TDa

Successfully measured a single bacteriophage T4

Abstract

The significance of mass spectrometry lies in its unparalleled ability to accurately identify and quantify molecules in complex samples, providing invaluable insights into molecular structures and interactions. Here, we leverage diamond nanostructures as highly sensitive mass sensors by utilizing a self-excitation mechanism under an electron beam in a conventional scanning electron microscope (SEM). The diamond molecular balance (DMB) exhibits an exceptional mass resolution of 0.36 MDa, based on its outstanding mechanical quality factor and frequency stability, along with an extensive dynamic range from MDa to TDa. This positions the DMB at the forefront of molecular balances operating at room temperature. Notably, the DMB demonstrates its ability to measure the mass of a single bacteriophage T4 by precisely locating the analyte on the device. These findings highlight the groundbreaking potential of the DMB as a revolutionary tool for mass spectrometry at room temperature.