Thousand foci coherent anti-Stokes Raman scattering microscopy

TL;DR

This paper presents a high-speed, megapixel coherent anti-Stokes Raman scattering (CARS) microscopy technique with 1089 foci, utilizing advanced laser sources and multichannel detection to achieve rapid, high-resolution chemical imaging.

Contribution

The authors introduce a novel CARS microscopy method with 1089 foci and multichannel detection, enabling significantly faster imaging speeds while maintaining sample scattering insensitivity.

Findings

Achieved 0.3Hz frame rate for megapixel CARS images.

Laser source enables at least 1000 times higher speed.

Potential for >100Hz megapixel CARS imaging with faster cameras.

Abstract

We demonstrate coherent anti-Stokes Raman scattering (CARS) microscopy with 1089 foci, enabled by a high repetition rate amplified oscillator and optical parametric amplifier. We employ a camera as multichannel detector to acquire and separate the signals from the foci, rather than using the camera image itself. This allows to retain the insensitivity of the imaging to sample scattering afforded by the non-linear excitation point-spread function, which is the hallmark of point-scanning techniques. We show frame rates of 0.3Hz for a megapixel CARS image, limited by the camera used. The laser source and corresponding CARS signal allows for at least 1000 times higher speed, and using faster cameras would allow acquiring at that speed, opening a perspective to megapixel CARS imaging with more than 100Hz frame rate.