Synchronization of E. coli bacteria moving in coupled wells

TL;DR

This study develops a microfluidic assay to observe and quantify synchronization in motile E. coli bacteria within coupled microcavities, revealing nonlinear dynamics and phase synchronization mechanisms.

Contribution

It introduces a novel single-cell assay for studying bacterial synchronization and provides a quantitative framework for understanding coupling in microbial motility.

Findings

E. coli exhibit self-sustained oscillations in microcavities.

Coupled bacteria show nonlinear synchronization with phase slips.

Mathematical rules quantify coupling strength.

Abstract

Synchronization plays a crucial role in the dynamics of living organisms, from fireflies flashing in unison to pacemaker cells that jointly generate heartbeats. Uncovering the mechanism behind these phenomena requires an understanding of individual biological oscillators and the coupling forces between them. Here, we develop a single-cell assay that studies rhythmic behavior in the motility of individual E.coli cells that can be mutually synchronized. Circular microcavities are used to isolate E.coli cells that swim along the cavity wall, resulting in self-sustained oscillations. Upon connecting these cavities by microchannels the bacterial motions can be coupled, yielding nonlinear dynamic synchronization patterns with phase slips. We demonstrate that the coordinated movement observed in coupled E. coli oscillators follows mathematical rules of synchronization which we use to quantify the coupling strength. These findings advance our understanding of motility in confinement, and lay the foundation for engineering desired dynamics in microbial active matter.