Characterizing virulence differences in a parasitoid wasp through comparative transcriptomic and proteomic

TL;DR

This study investigates the molecular basis of virulence differences in Cotesia typhae parasitoid wasps using comparative transcriptomic and proteomic analyses, revealing key gene expression patterns and candidate virulence factors.

Contribution

It provides the first detailed comparison of gene and protein expression related to virulence in two Cotesia typhae strains, highlighting molecular candidates linked to parasitism success.

Findings

Virulence genes are mainly expressed during the nymphal stage.

Differences in venom protein composition between strains.

Proviral gene expression varies temporally and between strains.

Abstract

Background: Two strains of the endoparasitoid Cotesia typhae present a differential parasitism success on the host, Sesamia nonagrioides. One is virulent on both permissive and resistant host populations, and the other only on the permissive host. This interaction provides a very interesting frame for studying virulence factors. Here, we used a combination of comparative transcriptomic and proteomic analyses to unravel the molecular basis underlying virulence differences between the strains.Results: First, we report that virulence genes are mostly expressed during the nymphal stage of the parasitoid. Especially, proviral genes are broadly up-regulated at this stage, while their expression is only expected in the host. Parasitoid gene expression in the host increases with time, indicating the production of more virulence factors. Secondly, comparison between strains reveals differences in venom composition, with 12 proteins showing differential abundance. Proviral expression in the host displays a strong temporal variability, along with differential patterns between strains. Notably, a subset of proviral genes including protein-tyrosine phosphatases is specifically over-expressed in the resistant host parasitized by the less virulent strain, 24 hours after parasitism. This result particularly hints at host modulation of proviral expression.Conclusions: This study sheds light on the temporal expression of virulence factors of Cotesia typhae, both in the host and in the parasitoid. It also identifies potential molecular candidates driving differences in parasitism success between two strains. Together, those findings provide a path for further exploration of virulence mechanisms in parasitoid wasps, and offer insights into host-parasitoid coevolution.