Cellular dynamics of host-parasitoid interactions: Insights from the encapsulation process in a partially resistant host

TL;DR

This study investigates the cellular immune response of a partially resistant host to parasitism by Cotesia typhae, revealing delayed encapsulation and potential passive immune evasion mechanisms.

Contribution

It provides new insights into the cellular dynamics and immune evasion strategies during host-parasitoid interactions in resistant hosts.

Findings

Parasitism delays encapsulation in resistant hosts.

Hemocyte counts increase with inert beads but not with parasitism.

Passive immune evasion may complement known virulence factors.

Abstract

Cotesia typhae is an eastern African endoparasitoid braconid wasp that targets the larval stage of the lepidopteran stem borer, Sesamia nonagrioides, a maize crop pest in Europe. The French host population is partially resistant to the Makindu strain of the wasp, allowing its development in only 40% of the cases. Resistant larvae can encapsulate the parasitoid and survive the infection. This interaction provides a very interesting frame for investigating the impact of parasitism on host cellular resistance. We characterized the parasitoid ovolarval development in a permissive host and studied the encapsulation process in a resistant host by dissection and histological sectioning compared to that of inert chromatography beads. We measured the total hemocyte count in parasitized and bead-injected larvae over time to monitor the magnitude of the immune reaction. Our results show that parasitism of resistant hosts delayed encapsulation but did not affect immune abilities towards inert beads. Moreover, while bead injection increased total hemocyte count, it remained constant in resistant and permissive larvae. We conclude that while Cotesia spp virulence factors are known to impair the host immune system, our results suggest that passive evasion could also occur.