Advancing Biomedical Applications: Antioxidant and Biocompatible Cerium Oxide Nanoparticle-Integrated Poly-{\epsilon}- caprolactone Fibers

TL;DR

This paper presents a new method for encapsulating cerium oxide nanoparticles in poly-{ extepsilon}-caprolactone fibers, reducing cytotoxicity and enhancing biocompatibility for biomedical applications like tissue engineering and wound healing.

Contribution

It introduces a novel encapsulation technique for nanoceria in PCL fibers, improving biocompatibility and efficacy for biomedical uses.

Findings

Encapsulation reduces ROS levels more effectively than direct nanoparticle administration.

PCL fibers with nanoceria show high biocompatibility in cell studies.

The system is promising for tissue engineering and targeted drug delivery.

Abstract

Reactive oxygen species (ROS), which are expressed at high levels in many diseases, can be scavenged by cerium oxide nanoparticles (CeO2NPs). CeO2NPs can cause significant cytotoxicity when administered directly to cells, but this cytotoxicity can be reduced if CeO2NPs can be encapsulated in biocompatible polymers. In this study, CeO2NPs were synthesized using a one-stage process, then purified, characterized, and then encapsulated into an electrospun poly-{\epsilon}-caprolactone (PCL) scaffold. The direct administration of CeO2NPs to RAW 264.7 Macrophages resulted in reduced ROS levels but lower cell viability. Conversely, the encapsulation of nanoceria in a PCL scaffold was shown to lower ROS levels and improve cell survival. The study demonstrated an effective technique for encapsulating nanoceria in PCL fiber and confirmed its biocompatibility and efficacy. This system has the potential to be utilized for developing tissue engineering scaffolds, targeted delivery of therapeutic CeO2NPs, wound healing, and other biomedical applications.